C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/138, 195/39
C12N 9/00 (2006.01) C12N 9/28 (2006.01) C12P 19/14 (2006.01)
Patent
CA 1081633
ABSTRACT OF THE DISCLOSURE Heat and acid-stable alpha-amylase enzymes having the following characteristics: (1) capable of retaining at least about 70% of their initial activity when held at 90°C and at a pH of 6.0 for 10 minutes in the absence of calcium ion; (2) capable of retaining at least about 50% of their initial activity when held at 90°C at a pH of 6.0 for 60 minutes in the absence of added calcium ion; (3) capable of retaining at least about 50% of their initial activity at a temperature of 80°C and at a pH of 4.55 in the presence of 5mM calcium ion for 10 minutes; and/or (4) capable of retaining at least about 95% of their initial activity at a temperature of 80°C at a pH ranging from about 4 to about 6.5. The preferred alpha-amylases are prepared by culturing a strain of a Bacillus stearothermophilus microorganism in a suitable culture medium. The novel alpha-amylases are useful in hydrolyzing and/or liquefying starch and due to their stability at low pH values they can be used in con- junction with other acid stable amylases such as gluco- amylase in either a soluble or an immobilized form.
276395
Ishii Yoshiko
Kanno Mutsuo
Tamura Masaki
Cpc International Inc.
Sherman
LandOfFree
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