Heat resistant maltose phosphorylase, process for...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 9/10 (2006.01) C12N 1/20 (2006.01) C12P 19/02 (2006.01) C12P 19/18 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2182059

This invention relates to heat resistant maltose phosphorylase having an activity of 80 % or more of the one untreated after treated in a buffer of pH 6.0, at one temperature of 50 to 60°C for 15 minutes, a process for preparation thereof, bacteria used for preparation thereof, and processes for preparation of .beta.-glucose-1 -phosphoric and trehalose using the enzyme. By carrying out enzymatic reaction at high reaction temperatures using this enzyme, it is possible to prepare .beta.-glucose-1- phosphoric acid or trehalose industrially advantageously, with lowering of contamination with various germs and shortening of reaction time.

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