Heat shock promoter and gene

C - Chemistry – Metallurgy – 12 – N

Patent

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195/1.2, 195/1.2

C12N 15/62 (2006.01) C07K 14/325 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 15/29 (2006.01) C12N 15/32 (2006.01) C12N 15/56 (2006.01) C12N 15/82 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 1338010

A class of genes known as heat shock or stress genes occurs in all organisms from bacteria to man. Transcription of these genes is initiated following a stress treatment (e.g., heat shock) and translation of the transcripts produces proteins that probably protect the cell temporarily. During stress, normal polyribosomes quickly break down to monoribosomes which are then used to translate the heat shock mRNA's. Normal mRNA's present before the stress treatment are in some way protected during the stress period and they can be re-used in translation following termination of the stress. The production of heat shock mRNA's and proteins is only a temporary phenomenon and the expression of the heat shock genes levels off after a few hours and then declines. If the temperature is increased slowly rather than in a single step, an organism can withstand temperatures which would otherwise be lethal, i.e., the organism can adapt to higher temperatures.

478916

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