Heat stable mutants of starch biosynthesis enzymes

C - Chemistry – Metallurgy – 12 – N

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C12N 15/54 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C12N 5/04 (2006.01) C12N 9/12 (2006.01) C12N 15/29 (2006.01) C12N 15/82 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2475540

The subject invention pertains to novel mutant polynucleotide molecules that encode enzymes that have increased heat stability. These polynucleotides, when expressed in plants, result in increased yield in plants grown under conditions of heat stress. The polynucleotide molecules of the subject invention encode maize endosperm ADP glucose pyrophosphorylase (AGP) and soluble starch synthase (SSS) enzyme activities. Plants and plant tissue bred to contain, or transformed with, the mutant polynucleotides, and expressing the polypeptides encoded by the polynucleotides, are also contemplated by the present invention. The subject invention also concerns methods for isolating polynucleotides and polypeptides contemplated within the scope of the invention. Methods for increasing yield in plants grown under conditions of heat stress are also provided.

L'invention concerne de nouvelles molécules polynucléotidiques mutantes qui codent des enzymes présentant une thermostabilité accrue. Ces polynucléotides, lorsqu'ils sont exprimés dans des plantes, ont pour résultat un rendement accru dans des plantes que l'on fait croître dans des conditions de thermostress. Les molécules polynucléotidiques de cette invention codent les activités de l'ADP-glucose pyrophosphorylase (AGP) d'endosperme de maïs et de l'enzyme de synthase d'amidon soluble (SSS). L'invention concerne également des plantes et des tissus créés pour contenir ces polynucléotides mutants ou transformés à l'aide de ces derniers, et exprimant les polypeptides codés par ces polynucléotides. L'invention concerne encore des procédés permettant d'isoler les polynucléotides et polypeptides relevant de cette invention. L'invention concerne enfin des procédés permettant d'augmenter le rendement dans des plantes que l'on fait croître dans des conditions de thermostress.

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