C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/14 (2006.01) C12N 9/12 (2006.01) C12N 15/01 (2006.01) C12N 15/29 (2006.01) C12N 15/54 (2006.01) C12N 15/82 (2006.01) C12P 21/02 (2006.01)
Patent
CA 2441207
The subject invention pertains to novel mutant polynucleotide molecules that encode enzymes that have increased heat stability. These polynucleotides, when expressed in plants, result in increased yield in plants grown under conditions of heat stress. In one embodiment, the polynucleotide molecules of the subject invention encode maize endosperm ADP glucose pyrophosphorylase (AGP) and soluble starch synthase (SSS) enzyme activities. Plants and plant issue bred to contain, or transformed with, the mutant polynecleotides, and expressing the polypeptides encoded by the polynucleotides, are also contemplated by the present invention. The subject invention also concerns methods for isolating polynucleotides and polypeptides contemplated within the scope of the invention. Methods for increasing yield in plants grown under conditions of heat stress are also provided.
L'invention concerne de nouvelles molécules mutantes de polynucléotides qui codent pour des enzymes présentant une thermostabilité accrue. Lorsqu'ils sont exprimés dans des plantes, ces polynucléotides assurent un rendement de culture accru obtenue avec les plantes cultivées dans des conditions de stress thermique. Dans l'un des modes de réalisation de l'invention, les molécules de polynucléotides décrites codent les activités enzymatiques ADP glucose pophosphorylase (AGP) et synthase d'amidon soluble (SSS: soluble starch synthase) de l'endosperme du maïs. L'invention concerne également les plantes et les tissus végétaux produits pour contenir les polynucléotides mutants, ou transformés avec ces derniers, et exprimant les polypeptides codés par ces polynucléotides. L'invention concerne, en outre, des procédés permettant d'isoler ces polynucléotides et polypeptides ainsi que des procédés permettant d'accroître le rendement de culture pour des plantes cultivées dans des conditions de stress thermique.
Burger Brian
Greene Thomas W.
Hannah L. Curtis
Mbm Intellectual Property Law Llp
University Of Florida Research Foundation Inc.
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