Helicobacter pylori with modified lipopolysaccharide...

A - Human Necessities – 61 – K

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A61K 39/106 (2006.01) A61P 31/04 (2006.01) C07K 14/205 (2006.01) C12N 1/20 (2006.01) C12N 9/10 (2006.01) C12N 15/31 (2006.01)

Patent

CA 2415586

Helicobacter pylori can be manipulated at a genetic level to prevent the synthesis of auto-reactive LPS, without destroying the physical integrity or function of the bacterial outer membrane. The bacterium is particularly useful for preparing cellular vaccines which do not induce antibodies which cross- react with self epitopes. Cellular H.pylori vaccines are provided which comprise mutant H.pylori which either does not express LPS or, preferably, expresses a mutant form of LPS.

L'invention concerne la manipulation génétique de Helicobacter pylori afin d'empêcher la synthèse des lipopolysaccharides (LPS) auto-réactifs sans détruire l'intégrité physique ou la fonction de la membrane extérieure bactérienne. Cette bactérie est particulièrement utile dans la préparation de vaccins cellulaires n'induisant pas d'anticorps à réaction croisée avec des auto-épitopes. L'invention concerne également des vaccins cellulaires contre H.pylori comprenant un H.pylori mutant n'exprimant pas les LPS ou exprimant, de préférence, une forme mutante de LPS.

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