Helper virus-free aav production

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 7/01 (2006.01) C12N 5/10 (2006.01) C12N 7/02 (2006.01) C12N 15/864 (2006.01)

Patent

CA 2221293

A method for the production of adeno-associated virus stocks and recombinant adeno-associated virus stocks that are substantially free of contaminating helper virus is described. The method utilizes transfection with helper virus vectors to replace the infection with helper virus used in the conventional method.

Cette invention concerne un procédé de production de stocks de virus adéno-associés et de stocks de virus adéno-associés recombinés qui ne contiennent sensiblement pas de virus auxiliaire contaminant. Ce procédé fait appel à la transfection à l'aide de vecteurs de virus auxiliaires, afin de remplacer l'infection effectuée à l'aide de virus auxiliaire et utilisée dans le procédé classique.

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