Helper virus-free herpes virus amplicon particles and uses...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 7/00 (2006.01) A61K 35/12 (2006.01) A61K 35/76 (2006.01) A61K 39/00 (2006.01) A61K 39/245 (2006.01) A61P 31/00 (2006.01) A61P 35/00 (2006.01) C07K 14/035 (2006.01) C07K 14/705 (2006.01) C12N 5/10 (2006.01) C12N 7/04 (2006.01) C12N 15/86 (2006.01) C12N 15/869 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2430589

The invention features new helper virus-free methods for making herpesvirus amplicon particles that can be used in immunotherapies, including those for treating any number of infectious diseases and cancers (including chronic lymphocytic leukemia, other cancers in which blood cells become malignant, lymphomas (e.g. Hodgkin's lymphoma or non-Hodgkin's type lymphomas). Described herein are methods of making helper virus-free HSV amplicon particles; cells that contain those particles (e.g, packaging cell lines or patients' cells, infected in vivo or ex vivo ; particles produced according to those methods; and methods of treating a patient with an hf-HSV particle made according to those methods.

L'invention concerne des nouvelles méthodes de production de particules d'amplicon d'herpèsvirus pouvant être utilisées dans les immunothérapies, dont celles destinées à traiter des maladies infectieuses et des cancers (dont la leucémie lymphocytique chronique, d'autres cancers dans lesquels les cellules sanguines deviennent malignes, les lymphomes comme, par exemple, la maladie de Hodgkin ou les lymphomes autres que la maladies de Hodgkin). L'invention porte sur des méthodes de fabrication de particules d'amplicon de HSV exemptes de virus assistant, sur des cellules contenant lesdites particules (ex : les lignées d'encapsidation les cellules de patients, infectées in vivo ou ex vivo), sur des particules produites selon lesdites méthodes et sur des méthodes de traitement d'un patient à l'aide d'une particule de hf-HSV produite selon lesdites méthodes.

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