Hemangio colony forming cells and non-engrafting hemangio cells

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/071 (2010.01) C12N 5/0789 (2010.01) A61K 35/12 (2006.01) A61K 35/44 (2006.01) A61P 9/10 (2006.01)

Patent

CA 2722693

Methods of generating and expanding human hemangio-colony forming cells and non-engrafting hemangio cells in vitro and methods of expanding and using such cells are disclosed. The methods permit the production of large numbers of hemangio-colony forming cells, non-engrafting hemangio cells as well as derivative cells, such as hematopoietic and endothelial cells. The cells obtained by the methods disclosed may be used for a variety of research, clinical, and therapeutic applications. Human non-engrafting hemangio cells are a novel progenitor cell population that is related to but distinct from the hemangioblast and human hemangio-colony forming cells. The invention also provides compositions, preparations, and solutions comprising hemangio-colony forming cells, non-engrafting hemangio cells or cells differentiated therefrom. The compositions, preparations, and solutions include cryopreserved preparations and substantially purified preparations, as well as mixed compositions formulated in combination with related hemangioblast progenitor cell types that can engraft into the bone marrow.

La présente invention concerne des procédés de génération et de multiplication de cellules formant des colonies hémangioblastiques humaines et de cellules hémangioblastiques non transplantables in vitro, ainsi que des procédés de multiplication et d'utilisation desdites cellules. Ces procédés permettent la production d'un grand nombre de cellules formant des colonies hémangioblastiques, de cellules hémangioblastiques non transplantables, ainsi que de cellules dérivées, telles que des cellules hématopoïétiques et endothéliales. Les cellules obtenues par les procédés de l'invention peuvent être utilisées pour diverses applications cliniques, thérapeutiques et de recherche. Les cellules hémangioblastiques non transplantables constituent une population inédite de cellules progénitrices proches, mais distinctes, des hémangioblastes et des cellules formant des colonies hémangioblastiques humaines. L'invention concerne également des compositions, des préparations et des solutions comprenant des cellules formant des colonies hémangioblastiques, des cellules hémangioblastiques non transplantables ou des cellules différenciées à partir des précédentes. Lesdites compositions, préparations et solutions comprennent des préparations cryoconservées et des préparations essentiellement purifiées, ainsi que des compositions mixtes dans lesquelles lesdites cellules de l'invention sont associées à des types de cellules progénitrices hémangioblastiques qui peuvent être transplantées dans la moelle osseuse.

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