Hematopoietic stem cells and methods for generating such cells

Details Hematopoietic stem cells and methods for generating such cells Hematopoietic stem cells and methods for generating such cells

C - Chemistry, Metallurgy

12

N

C12N 5/10 (2006.01) A61K 31/70 (2006.01) A61K 35/28 (2006.01) A61K 48/00 (2006.01) C07K 14/47 (2006.01) C12N 5/06 (2006.01) C12N 15/85 (2006.01) C12P 21/00 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2267288

The present invention offers a developmental hematopoietic system which can be used to reproducibly and efficiently develop hematopoietic cells with a well- defined kinetic pattern. In one aspect, the method of the present invention generally includes the steps of obtaining viable stem cells from an animal donor and transfecting the cells with an expression construct including a hematopoietic gene, such as the LH-2 gene, to produce viable hematopoietic stem cells.

Système de développement hématopoïetique susceptible d'être utilisé pour la production, dans des conditions reproductibles et efficaces, de cellules hématopoïetiques ayant un type cinétique bien défini. En particulier, on décrit un procédé qui consiste généralement à recueillir des cellules souches viables auprès d'un animal donneur et à conduire la transfection de ces cellules avec un produit d'expression de recombinaison incluant un gène hématopoïetique (par exemple, gène LH-2), afin de produire des cellules souches hématopoïetiques viables.

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