C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/0789 (2010.01) A61K 35/14 (2006.01) A61P 7/00 (2006.01) C12N 5/00 (2006.01)
Patent
CA 2522743
A method of in vitro fucosylation of selectin ligands on cord blood-derived hematopoietic stem cells for bone marrow transplantation is disclosed. In this method, an effective amount of an .alpha.1,3-fucosyltransferase, e.g., .alpha.1,3-fucosyltransferase VI, is used in vitro to treat cord blood-derived hematopoietic stem cells to convert non-functional PSGL-1 or other ligands on the cell surface into functional forms that bind selectins, especially P- selectin or E-selectin. The treated cells have enhanced effectiveness in reconstituting bone marrow in patients in need of such therapy.
La présente invention concerne un procédé de fucosylation in vitro de ligands de sélectine sur des cellules souches hématopoïétiques provenant du sang ombilical, permettant d'effectuer une transplantation de moelle osseuse. Selon ce procédé, une quantité efficace d'une a1,3-fucosyltransférase, par exemple a1,3-fucosyltransférase VI, est utilisée in vitro afin de traiter des cellules souches hématopoïétiques provenant du sang ombilical, en vue de transformer des ligands PSGL-1 non fonctionnels ou d'autres ligands à la surface de la cellule sous des formes fonctionnelles qui se lient à des sélectines, notamment la P-sélectine ou la E-sélectine. Les cellules traitées sont plus efficaces pour reconstruire la moelle osseuse chez des patients nécessitant une telle thérapie.
Mcever Rodger P.
Xia Lijun
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Mcever Rodger P.
Xia Lijun
LandOfFree
Hematopoietic stem cells treated by in vitro fucosylation... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Hematopoietic stem cells treated by in vitro fucosylation..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Hematopoietic stem cells treated by in vitro fucosylation... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1948024