Hemoglobin derivative measurement method, and reagent...

G - Physics – 01 – N

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G01N 33/72 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2604474

The amount of a hemoglobin derivative in a sample can be determined by treating a sample solution containing the hemoglobin derivative with a denaturation reagent comprising a nonionic surfactant and an oxidizing agent to denaturalize the hemoglobin derivative in the sample solution and then performing the immunoassay utilizing an antibody specific to the denaturalized site on the hemoglobin derivative. In this method, it can be ensured the rapid denaturation of hemoglobin while minimizing the influence of a degeneration reagent on an immunological reaction during the determination of a hemoglobin derivative.

L'invention concerne la détermination de la quantité d'un dérivé d'hémoglobine dans un échantillon par traitement d'une solution d'échantillon contenant le dérivé d'hémoglobine avec un réactif de dénaturation comprenant un agent de surface non ionique et un agent oxydant afin de dénaturer le dérivé d'hémoglobine dans la solution d'échantillon. On procède ensuite à un immunodosage à l'aide d'un anticorps spécifique au site dénaturé sur le dérivé d'hémoglobine. Ledite méthode garantit la dénaturation rapide de l'hémoglobine tout en minimisant l'influence d'un réactif de dégénération sur une réaction immunoloique au cours de la détermination du dérivé d'hémoglobine.

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