Hemoglobin mutants with increased soluble expression and/or...

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 14/805 (2006.01) C12N 1/21 (2006.01) C12N 15/12 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2299049

The invention relates to novel recombinant hemoglobins having reduced nitric oxide scavenging and/or increased high soluble expression. The invention further relates to methods of increasing the soluble expression of recombinant hemoglobin by adding exogenous hemin in molar excess of the heme binding sites of recombinant hemoglobin.

L'invention concerne de nouvelles hémoglobines recombinantes ayant une évacuation réduite d'oxyde nitrique et/ou une expression soluble accrue. L'invention concerne également des procédés permettant d'augmenter l'expression soluble de l'hémoglobine recombinante par ajout d'hémine exogène en excès molaire des sites de liaison hème de l'hémoglobine recombinante.

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