Heparin coating of biological tissue

A - Human Necessities – 61 – K

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Details

A61K 31/727 (2006.01) C12N 5/071 (2010.01) A61K 35/12 (2006.01) A61K 35/39 (2006.01) A61P 37/06 (2006.01)

Patent

CA 2612621

The present invention discloses an in vitro method to provide biological t issue with a heparin coating comprising the following steps; linking a biotin reagent to biological tissue, linking an avidin reagent to the biotinylated biological tissue, and linking a heparin reagent to the formed layer of avidin on the biological t issue thus forming a heparin coating. The invention further discloses a heparin coating on biological t issue, the use of a heparin coating, and the biological t issue coated with a heparin layer according to the method.

La présente invention décrit un procédé in vitro pour obtenir un tissu biologique avec un revêtement d~héparine qui comprend les étapes suivantes : la liaison d~un réactif à base de biotine à un tissu biologique, la liaison d~un réactif à base d~avidine au tissu biologique biotinylé et la liaison d~un réactif à base d~héparine à la couche formée d~avidine sur le tissu biologique, formant ainsi un revêtement d~héparine. L~invention décrit en outre un revêtement d'héparine sur un tissu biologique, l'utilisation d'un revêtement d'héparine et le tissu biologique revêtu d'une couche d'héparine selon le procédé.

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