Heparinase gene from flavobacterium heparinum

Details Heparinase gene from flavobacterium heparinum Heparinase gene from flavobacterium heparinum

C - Chemistry, Metallurgy

12

N

C12N 15/60 (2006.01) C12N 1/21 (2006.01) C12N 9/88 (2006.01)

Patent

CA 2122004

The cloning of the heparinase gene from Flavobacterium Heparinum using the polymerase chain reaction is described. The Open Reading Frame (ORF) corresponded to 1152 base pairs encoding a precursor protein of MW 43,800 daltons. The amino ac- id sequence reveals a 20-residue leader peptide. The gene was expressed in two expression systems in E. Coli.

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