Hepatitis a virus purified and triply cloned

Details Hepatitis a virus purified and triply cloned Hepatitis a virus purified and triply cloned

C - Chemistry, Metallurgy

12

N

167/130

C12N 7/08 (2006.01) A61K 39/29 (2006.01) A61K 39/00 (2006.01)

Patent

CA 1260392

ABSTRACT OF THE DISCLOSURE Human hapatitis A virus (HAV) can be purified by a process in which master seed lots of HM-175 strain of hepatitis A virus are prepared from triply cloned virus by terminal dilution at passage level about 20 or preferably at least 20-30. The clones tested induced minimal or no hepatitis although significant antibody response was provoked in inoculated primates. A method of preparing the triply cloned inoculum is also described.

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