Hepatitis c virus ns2/3 activity assay

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/37 (2006.01) C12N 9/48 (2006.01) C12N 15/57 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2627247

The invention provides a novel assay for NS2/3 cleavage detection, particularly, a novel assay for the detection of the NS3 cleavage product in the presence of uncleaved NS2/3 based on discrimination of the activity of the resulting NS3 protease product from the NS3 protease activity of the NS2/3 uncleaved protein. Certain reaction conditions allow this discrimination in situ without having to resort to physical separation of both proteases by decreasing the NS3 protease activity of the NS2/3 protein and increasing the NS3 protease activity of the resulting NS3 protease product, thereby producing a "signal window" upon which it is feasible to distinguish and measure NS3 protease activity produced from the cleavage of NS2/3.

L'invention concerne une nouvelle analyse utilisée dans la détection du clivage de NS2/3, notamment, dans la détection du produit de clivage de NS3 en présence de NS2/3 non clivée sur la base de la discrimination de l'activité du produit résultant de la protéase NS3 provenant de l'activité de la protéase NS3 de la protéine non clivée NS2/3. Certaines conditions de réaction permettent cette discrimination in situ sans avoir à recourir à la séparation physique des deux protéases en réduisant l'activité de la protéase NS3 du produit résultant de la protéase NS3, ce qui permet d'obtenir une "fenêtre de signal" sur laquelle il est facile de distinguer et de mesurer l'activité de la protéase NS3 produite par le clivage de NS2/3.

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