C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 16/10 (2006.01) C12Q 1/37 (2006.01) G01N 33/53 (2006.01) G01N 33/566 (2006.01) C07K 7/06 (2006.01) C12Q 1/70 (2006.01)
Patent
CA 2539846
The present invention provides a novel assay for the detection of the NS2/3 cleavage products NS2 or NS3 in the presence of uncleaved NS2/3. In the present invention, following self-cleavage of NS2/3 to generate NS2 and NS3 cleavage products, the sample is incubated with a ligand specific for the recognition of NS2 or NS3 cleavage product in the presence of uncleaved NS2/3. There is provided a method for detecting a NS2/3 autocleavage product in a sample containing NS2/3 protease, the assay comprising: a) subjecting the sample to suitable conditions under which at least a portion of the NS2/3 protease is self-cleaved to yield cleaved NS2 and NS3 products; b) incubating the NS2 and NS3 products with a ligand that can preferentially bind to one of either NS2 or NS3 product over NS2/3 under conditions suitable to afford binding of the ligand to NS2 or NS3; and c) detecting NS2- or NS3-bound ligand produced in step b), whereby the amount of bound ligand detected correlates with the NS2/3 autocleavage activity. The present method is also useful as an assay to screen candidate NS2/3 inhibitor compounds as potential treatment for HCV infection. A further aspect of the present invention concerns ligands selectively recognizing one of the NS2 cleavage product or the NS3 cleavage product with minimal cross- reactivity with the uncleaved NS2/3 and the other cleaved product. Specifically, the present invention provides antibodies that selectively recognize one of cleaved NS2 product or cleaved NS3 product with minimal cross-reactivity with the uncleaved NS2/3 and the other cleaved product.
Lagace Lisette
Lamarre Lyne
Thibeault Diane
Boehringer Ingelheim International Gmbh
Looper Ywe J.
LandOfFree
Hepatitis c virus ns2/3 assay does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Hepatitis c virus ns2/3 assay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Hepatitis c virus ns2/3 assay will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1573235