Hepatitis g virus and molecular cloning thereof

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/40 (2006.01) A61K 39/29 (2006.01) C07H 21/00 (2006.01) C07K 7/08 (2006.01) C07K 14/18 (2006.01) C07K 16/10 (2006.01) C12N 7/00 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) G01N 33/576 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2190860

Polypeptide antigens are disclosed which are immunoreactive with sera from individuals having a non-A, non-B, non-C, non-D, non-E Hepatitis, herein designated Hepatitis G virus (HGV). Corresponding genomic-fragment clones containing polynucleotides encoding the open reading frame sequences for the antigenic polypeptides are taught. The antigens are useful in diagnostic methods for detecting the presence of HGV in test subjects. The antigens are also useful in vaccine and antibody preparations. In addition, the entire coding sequences of two HGV isolates are disclosed. Methods are presented for nucleic acid-based detection of HGV in samples and also methods for the isolation of further genomic sequences corresponding to HGV.

L'invention concerne des antigènes polypeptidiques qui sont imunoréactifs avec des sérums provenant de personnes ayant une hépatite non A, non B, non C, non D, non E, désignée ici virus de l'hépatite G (HGV). Des clones de fragments génomiques correspondants contenant des polynucléotides codant les séquences de structures à lecture directe provenant de polypeptides antigéniques sont également concernés. Les antigènes sont utiles dans des procédés diagnostics pour la détection de la présence du HGV dans des sujets soumis aux tests. Les antigènes sont également utiles dans des préparations de vaccins et d'anticorps. De plus, toutes les séquences de codage des deux isolats HGV sont décrites. L'invention décrit également la détection basée sur l'acide nucléique de HGV dans des échantillons, ainsi que des procédés permettant d'isoler d'autres séquences génomiques correspondant à HGV.

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