Herpes simplex virus icp4 is an inhibitor of apoptosis

A - Human Necessities – 61 – K

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A61K 48/00 (2006.01) A61K 31/70 (2006.01) A61K 38/16 (2006.01) A61K 39/42 (2006.01) C07K 14/035 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2262013

The ICP4 protein of herpes simplex virus plays an important role in the transactivation of viral genes. The present invention discloses that ICP4 also has the ability to inhibit apoptosis. This function appears to reside in functional domain distinct from the transactivating function, as indicated by studies using temperature sensitive mutants of ICP4 that transactivating function at elevated temperatures. Also disclosed are methods for inhibition of apoptosis using ICP4 or an ICP4 encoding gene, such as an .alpha.4 gene, methods of inhibiting ICP4's apoptosis-inhibiting function, and methods for the production of recombinant proteins and treatment of HSV infections.

La protéine ICP4 du virus de l'herpès simplex joue un rôle important dans la transactivation des gènes viraux. L'invention dévoile le fait que ICP4 possède également une capacité d'inhibition de l'apoptose. Cette fonction semble résider dans un domaine fonctionnel distinct de la fonction de transactivation, comme l'ont indiqué des études ayant mis en application des mutants sensibles à la température de ICP4 présentant cette fonction de transactivation à température élevée. L'invention concerne également des procédés servant à inhiber l'apoptose au moyen d'ICP4 ou d'un gène codant ICP4, tel qu'un gène .alpha.4, des procédés servant à inhiber la fonction d'inhibition de l'apoptose de ICP4, ainsi que des procédés servant à produire des protéines de recombinaison et de traitement d'infections par HSV.

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