Heterohybridomas producing human monoclonal antibodies to hiv-1

Details Heterohybridomas producing human monoclonal antibodies to hiv-1 Heterohybridomas producing human monoclonal antibodies to hiv-1

C - Chemistry, Metallurgy

12

P

C12P 21/08 (2006.01) C07K 14/16 (2006.01) C07K 16/10 (2006.01) C12N 5/10 (2006.01) C12N 5/26 (2006.01) C12N 5/28 (2006.01)

Patent

CA 2087347

Heterohybridomas producing human monoclonal antibodies against a neutralizing epitope of HIV-1 are prepared by transforming peripheral blood lymphocytes by Epstein-Barr virus, selecting cells producing an antibody to a synthetic peptide representing part of the neutralizing epitope, and fusing them with a heteromyeloma cell. A method for producing a human monoclonal antibody specific for a neutralizing HIV-1 epitope from the heterohybridoma is disclosed, as are human monoclonal antibodies specific for particular peptide epitopes of the V3 loop of the gp120 glycoprotein of HIV.

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