C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/42 (2006.01) C12N 15/56 (2006.01) C12P 7/10 (2006.01)
Patent
CA 2724076
The present invention provides for heterologous expression of polypeptides encoded by wild-type and codon-opti-mized variants of cbh1 and/or cbh2 from the fungal organisms Talaromyces emersonii (T. emersonii), Humicola grisea (H. grisea), Thermoascus aurantiacus (T. aurantiacus), and Trichoderma reesei (T. reesei) in host cells, such as the yeast Saccharomyces cere-visiae. The expression in such host cells of the corresponding genes, and variants and combinations thereof, result in improved specific activity of the expressed cellobiohydrolases. Thus, such genes and expression systems are useful for efficient and cost- ef-fective consolidated bioprocessing systems.
L'invention concerne l'expression hétérologue de polypeptides codés par des variants de type sauvage et optimisés par codon de cbhl et/ou cbh2 provenant d'organismes fongiques Talaromyces emersonii (T. emersonii), Humicola grisea (H. grisea), Thermoascus aurantiacus (T. aurantiacus), et Trichoderma reesei (T. reesei), dans des cellules hôtes telles que la levure Saccharomyces cerevisiae. L'expression des gènes correspondants, des variants et de leurs combinaisons, dans de telles cellules hôtes, se traduit par une activité spécifique améliorée des cellobiohydrolases exprimées. Ainsi, de tel gènes et systèmes d'expression sont utiles pour des systèmes de bio-traitement consolidés, efficaces et économiques.
Den Haan Riaan
Lagrange Danie
Van Zyl Emile
Gowling Lafleur Henderson Llp
Universiteit Stellenbosch
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