C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/56 (2006.01) C12N 1/19 (2006.01) C12N 9/42 (2006.01) C12N 15/81 (2006.01) C12P 7/10 (2006.01) C12P 19/14 (2006.01)
Patent
CA 2729945
The present invention provides for heterologous expression of termite and termite- associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost- effective consolidated bioprocessing systems.
La présente invention concerne lexpression hétérologue de cellulases de termite et de symbiote associé aux termites. Les cellulases peuvent, par exemple, être optimisées en terme de codons et exprimées dans des cellules hôtes de levure, telles que la levure Saccharomyces cerevisiae. Les cellulases peuvent également être co-exprimées dans des cellules hôtes avec dautres cellulases. Lexpression dans de telles cellules hôtes des cellulases de termite et de symbiote associé aux termites, et des variants et combinaisons de celles-ci, produit des levures ayant une activité cellulolytique améliorée. Par conséquent, de tels gènes et systèmes dexpression sont utiles pour des systèmes de bioproduction consolidés efficaces et économiques.
Brevnova Elena E.
Gandhi Chhayal
Mcbride John
Mellon Mark
Rajgarhia Vineet
Gowling Lafleur Henderson Llp
Mascoma Corporation
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