C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
530/15.14
C07K 14/52 (2006.01) A61K 35/22 (2006.01) A61K 38/00 (2006.01)
Patent
CA 1120468
ABSTRACT OF THE DISCLOSURE A colony-stimulating factor having definite physical and chemical properties and a function of stimu- lating activity on human bone marrow cells to proliferate and differentiate, thereby forming granulocyte colonies, is obtained from human urine by concentrating the urine with respect to proteins contained therein by adsorption chromatography with silica gel, salting out with ammonium sulfate and other means, then removing impurities by adsorption on cation exchanger, and further purifying by ion exchanging chromatography on anion exchanger, gel filtrating chromatography with highly crosslinked gels, affinity chromatography with sugar affinitire adsorbents and electrophoresis.
323547
Kuboyama Morio
Nishida Masayuki
Ogasa Katsuhiro
Saito Minoru
Takaku Fumimaro
Fetherstonhaugh & Co.
Ltd. Morinaga Milk Industry Co.
The Green Cross Corporation
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