High affinity oligonucleotide ligands to growth factors

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) A61K 31/70 (2006.01) C07H 21/00 (2006.01) C07K 14/49 (2006.01) C07K 14/495 (2006.01) C07K 14/50 (2006.01) C12N 15/11 (2006.01) G01N 33/53 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2221318

Methods are described for the identification and preparation of high-affinity nucleic acid ligands to TGF.beta., PDGF and hKGF. Included in the invention are specific RNA and ssDNA ligands to TGF.beta.1 and PDGF identified by the SELEX method. Also included in the invention are specific RNA ligands to hKGF identified by the SELEX method. Further included are RNA ligands that inhibit the interaction of TGF.beta.1 and hKGF with their receptors and DNA ligands that inhibit the interaction of PDGF with its receptor.

L'invention porte sur l'identification et la préparation d'acides nucléiques se comportant comme des ligands avec une affinité élevée pour le facteur transformant de croissance, TGF.beta., le facteur de croissance plaquettaire, PDGF et le facteur de croissance kérotinocytique humain, hKGF. L'invention porte en particulier sur des ARN et des ADN à un seul brin se comportant comme des ligands spécifiques pour TGF.beta.1 et pour PDGF, identifiés par la méthode SELEX, sur des ARN se comportant comme des ligands spécifiques pour hKGF, identifiés par la méthode SELEX, sur des ARN se comportant comme des ligands inhibant l'interaction de TGF.beta.1 et de hKGF avec leurs récepteurs et sur des ADN se comportant comme des ligands inhibant l'interaction de PDGF avec son récepteur.

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