High-cell density fed-batch fermentation process for...

Details High-cell density fed-batch fermentation process for... High-cell density fed-batch fermentation process for...

C - Chemistry, Metallurgy

12

P

C12P 21/02 (2006.01) C12N 1/20 (2006.01) C12N 15/00 (2006.01) C07K 14/22 (2006.01)

Patent

CA 2657135

Methods for producing proteins, for example, recombinant meningococcal 2086 proteins, using fed-batch fermentation with continuous input of an inducer after achieving a threshold parameter, and optionally continuous input of a carbon source, for example, a constant rate input, to improve protein yields, as well as high density protein compositions and compositions for use in the methods of the present invention, are provided.

L'invention concerne des procédés pour obtenir des protéines, par exemple, des protéines 2086 méningococciques recombinantes, par fermentation par lot alimenté avec introduction continue d'un inducteur après obtention d'un paramètre de seuil et, facultativement, introduction continue d'une source de carbone, par exemple introduction à vitesse constante, afin d'améliorer les rendements en protéines; l'invention concerne également des compositions de protéines à densité élevée et des compositions destinées à être utilisées dans les procédés de la présente invention.

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