High-copy-number, high-expression vector having methionine...

Details High-copy-number, high-expression vector having methionine... High-copy-number, high-expression vector having methionine...

C - Chemistry, Metallurgy

12

N

C12N 15/63 (2006.01) C07K 14/765 (2006.01) C07K 14/805 (2006.01) C12N 9/50 (2006.01) C12N 15/09 (2006.01) C12N 15/66 (2006.01) C12N 15/70 (2006.01) C12P 21/02 (2006.01) C12N 1/21 (2006.01)

Patent

CA 2610780

Disclosed is a high-copy-number, high-expression vector capable of producing a protein having a satisfactory function/activity in the same level as that of a natural form of the protein, in a large quantity and in a simple manner. A vector having (A) a target gene or a cloning site of the target gene, (B) a sequence element necessary for the high copying of the target gene, (C) a sequence element necessary for the expression of the target gene and a methionine aminopeptidase gene; a method for producing the vector; a transformant having the vector introduced therein; and a process for producing a protein using the transformant.

L'invention concerne un nombre de reproduction élevé, un vecteur d'expression élevée capable de produire facilement et en grande quantité une protéine présentant une fonction/activité satisfaisante de niveau équivalent à celui d'une forme naturelle de la protéine. L'invention porte sur un vecteur comprenant (A) un gène cible ou un site de clonage du gène cible, (B) un élément séquence indispensable pour la reproduction élevée du gène cible, (C) un élément séquence indispensable à l'expression du gène cible et d'un gène méthionine aminopeptidase; un procédé de production du vecteur; un transformant dans lequel le vecteur a été introduit; et un procédé permettant de produire une protéine en se servant du transformant.

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