C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) B01D 15/08 (2006.01) B01D 21/26 (2006.01) B03C 1/30 (2006.01) C07K 1/22 (2006.01) C12Q 1/68 (2006.01) G01N 1/34 (2006.01) G01N 35/00 (2006.01)
Patent
CA 2203295
Methods for separating target particles from a test medium in which the particles are suspended. The method contemplates utilization of blocking or masking particles which block or mask unwanted biological debris which tends to interfere with formation of complexes by which the target particles can be isolated. After centrifugation, the masking particles create a barrier between unwanted debris and test medium containing the target particles which permits the particles to be manipulated or modified without removing them from the debris. In a preferred aspect, magnetic separation techniques are used. In this aspect, non-magnetic 0.04 micron carboxylated polystyrene blocking particles are employed.
Méthodes pour séparer des particules cibles provenant d'un milieu expérimental dans lequel les particules se trouvent en suspension. La méthode vise l'utilisation de particules de blocage ou de masquage, pour bloquer ou masquer les débris biologiques indésirables qui ont tendance à interférer avec la formation des complexes permettant d'isoler les particules cibles. Après centrifugation, les particules de masquage créent une barrière entre les débris indésirables et le milieu d'essai renfermant les particules cibles, ce qui permet de manipuler ou de modifier les particules sans les enlever des débris. Dans une version privilégiée de l'invention, on fait appel à des techniques de séparation magnétique, avec utilisation de particules de blocage de 0,04 micron à base de polystyrène carboxylé.
Brisco Paula R.g.
Ekenberg Steven J.
Oaklander Anne Louise
Promega Corporation
Shapiro Cohen
The Johns Hopkins University School Of Medicine
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