High-expression vector for animal cells

Details High-expression vector for animal cells High-expression vector for animal cells

C - Chemistry, Metallurgy

12

N

C12N 15/85 (2006.01) C12N 5/10 (2006.01) C12N 15/09 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01) C12P 21/02 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2514941

It is intended to provide an overexpression vector which makes it possible to easily acquire a high-level producing strain with the use of mammalian cells, in particular, Chinese hamster ovary cells (CHO cells) as a host. Namely, an expression vector capable of inducing high productivity of a genetically modified protein in animal host cells which comprises a promoter strongly inducing expression, a multicloning site for gene integration and a polyadenylation signal sequence, in this order starting from the upstream, and a drug-resistance gene having no promoter functioning in animal cells in the downstream thereof.

L'invention concerne un vecteur de surexpression permettant d'acquérir facilement une souche de production élevée, au moyen de l'utilisation de cellules de mammifères, en particulier, de cellules d'ovaire de hamster chinois (cellules CHO) en tant qu'hôte. L'invention concerne un vecteur d'expression pouvant induire une haute productivité d'une protéine génétiquement modifiée dans des cellules hôtes animales, comprenant un promoteur induisant fortement une expression, un site multiclonage pour une intégration génique et une séquence de signal de polyadénylation, dans cet ordre, en commençant à l'amont, et un gène résistant au médicament ne présentant pas de promoteur, fonctionnant dans des cellules animales, en aval de celles-ci.

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