High fidelity detection of nucleic acid differences by...

Details High fidelity detection of nucleic acid differences by... High fidelity detection of nucleic acid differences by...

C - Chemistry, Metallurgy

12

N

C12N 9/00 (2006.01) C07H 21/00 (2006.01) C07K 14/82 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2260818

Ligase detection reaction is utilized to distinguish minority template in the presence of an excess of normal template with a thermostable ligase. This process can be carried out with a mutant ligase, thermostable ligase, or a modified oligonucleotide probe. This procedure is particularly useful for the detection of cancer-associated mutations. It has the advantage of providing a quantitative measure of the amount or ratio of minority template.

La réaction de détection par ligase est utilisée pour distinguer des matrices minoritaires en présence d'un excédent de matrices normales à l'aide d'une ligase thermostable. Ledit procédé peut être mis en oeuvre avec une ligase mutante, une ligase thermostable ou une sonde oligonucléotidique modifiée. Il est particulièrement utile pour détecter des mutations associées au cancer. Il présente l'avantage de fournir une mesure de la quantité ou du pourcentage de matrice minoritaire.

Affiliated with

Also associated with

Rating

High fidelity detection of nucleic acid differences by... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with High fidelity detection of nucleic acid differences by..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and High fidelity detection of nucleic acid differences by... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1483899