High fidelity dna polymerase compositions and uses therefor

Details High fidelity dna polymerase compositions and uses therefor High fidelity dna polymerase compositions and uses therefor

C - Chemistry, Metallurgy

12

N

C12N 15/54 (2006.01) C07H 21/04 (2006.01) C07K 1/00 (2006.01) C12N 9/00 (2006.01) C12N 9/12 (2006.01) C12N 9/22 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2471473

The subject invention relates to compositions comprising an enzyme mixture which comprises a first enzyme and a second enzyme; where the first enzyme comprises a DNA polymerization activity and the second enzyme comprises an 3'- 5' exonuclease activity and a reduced DNA polymerization activity. The invention also relates to the above compositions in kit format and methods for high fidelity DNA synthesis using the subject compositions of the invention.

La présente invention concerne des compositions à base d'un mélange de deux enzymes. La 1?ère¿ enzyme fait preuve d'une activité de polymérisation de l'ADN, la 2?ème¿ enzyme d'une activité exonucléase en 3'-5' ainsi que d'une activité réduite de polymérisation de l'ADN. L'invention concerne également des nécessaires à base de ces compositions, et des procédés de synthèse haute fidélité de l'ADN par utilisation des compositions de l'invention.

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