High-fidelity dna sequencing using solid phase capturable...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2442862

This invention provides methods for sequencing DNA by detecting the identity of a nucleotide within a DNA sequencing fragment using mass spectrometry. The invention provides cleavable linkers for attaching a label to a dideoxynucleotide and provides labeled dideoxynucleotides. The invention also provides methods for increasing mass spectrometry resolution using linkers with different mass. The invention further provides systems for separating a labeled moiety from non-labeled components in one or more samples in solution.

La présente invention concerne des procédés de séquençage de l'ADN par détection de l'identité d'un nucléotide dans un fragment de séquençage de l'ADN par spectrométrie de masse. L'invention concerne également, d'une part des lieurs clivables permettant de fixer une étiquette à un didésoxynucléotide, et d'autre part des didésoxynucléotides étiquetés. L'invention concerne aussi des procédés permettant d'augmenter la résolution de la spectrométrie de masse par utilisation de lieurs de différentes masses. L'invention concerne enfin des systèmes permettant de séparer un fragment étiqueté d'une composante non étiquetée dans un ou plusieurs échantillons en solution.

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