High fidelity thermostable ligase and uses thereof

Details High fidelity thermostable ligase and uses thereof High fidelity thermostable ligase and uses thereof

C - Chemistry, Metallurgy

12

N

C12N 15/52 (2006.01) C12N 1/21 (2006.01) C12N 9/00 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2348776

The present invention is directed to a thermostable ligase having substantially higher fidelity than either T4 ligase or Thermus thermophilus ligase. The DNA molecule encoding this enzyme as well as expression systems and host cells containing it are also disclosed. The thermostable ligase of the present invention is useful in carrying out a ligase detection reaction process and a ligase chain reaction process.

Cette invention concerne une ligase thermostable présentant une fidélité nettement supérieure à celle de la T4 ligase ou de la ligase Thermus thermophilus. L'invention porte également sur la molécule d'ADN codant pour cet enzyme ainsi que pour les systèmes d'expression et les cellules hôtes qui la renferment. La ligase thermostable selon la présente invention convient pour la mise en oeuvre d'un processus de réaction de détection de ligase et d'un processus de réaction en chaîne de ligase.

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