High-level expression of recombinant antibody in a mammalian...

Details High-level expression of recombinant antibody in a mammalian... High-level expression of recombinant antibody in a mammalian...

C - Chemistry, Metallurgy

12

N

C12N 15/85 (2006.01) C07K 16/00 (2006.01) C12N 5/10 (2006.01) C12N 15/13 (2006.01) C12P 21/08 (2006.01)

Patent

CA 2606576

The invention relates to a mammalian host cell with a high-level expression of recombinant antibody containing a double-gene vector with gene optimized nucleotide sequences encoding the light and the heavy antibody chains, the double-gene vector itself and a process for enhancing the production of recombinant antibody in a mammalian host cell.

L'invention concerne une cellule hôte de mammifère présentant une expression de niveau élevé d'un anticorps recombinant contenant un vecteur à gène double présentant des séquences nucléotidiques géniques optimisées codant les chaînes d'anticorps légères et les chaînes d'anticorps lourdes. L'invention concerne également le vecteur à gène double lui-même et un procédé pour accroître la production d'un anticorps recombinant dans une cellule hôte de mammifère.

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