High level of expression of ingap

Details High level of expression of ingap High level of expression of ingap

C - Chemistry, Metallurgy

12

N

C12N 15/63 (2006.01) A61K 38/17 (2006.01) C07K 1/22 (2006.01) C07K 14/47 (2006.01) C07K 14/475 (2006.01) C12N 1/21 (2006.01) C12N 15/00 (2006.01) C12P 21/02 (2006.01) C12N 15/12 (2006.01)

Patent

CA 2270412

Removal of the nucleotide sequence encoding the signal peptide from the INGAP coding sequence allows bacteria to express substantial amounts of INGAP activity. Previous attempts have provided only low yields of INGAP, possibly because the signal sequence of INGAP is toxic to bacteria.

La présente invention concerne l'élimination de la séquence de nucléotides codant pour le peptide signal à partir de la séquence de codage de la protéine INGAP, permettant à des bactéries d'exprimer de fortes quantités d'activité de la protéine INGAP. Des tentatives antérieures n'ont fourni que des rendements faibles du fait que, vraisemblablement, la séquence signal de l'INGAP est toxique pour des bactéries.

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