High loading of immobilized enzymes on activated carbon...

C - Chemistry – Metallurgy – 12 – N

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C12N 11/14 (2006.01) C12N 11/06 (2006.01)

Patent

CA 1129358

ABSTRACT OF THE DISCLOSURE Enzymes are immobilized on activated carbon supports at high load levels and with high stability by a procedure whereby a carbon support is first activated with a water-soluble carbodiimide derivative which forms a highly reactive intermediate with carboxyl and other active organic radicals on the surface of the carbon support; thereafter the complex of the carbon and carbodiimide is treated with an enzyme solution whereby the enzyme displaces the quaternary ammonium group and forms a carbon- enzyme complex wherein the enzyme is immobilized and yet the carbon retains its surface activity. Preferred immobilizing agents are quaternary ammonium forms of aminocarbodiimides or hydrochloric acid salts of water-soluble carbodiimides. The immobilization of the enzymes on activated carbon provides materials which are easily handled and which are stabilized against denaturation by hydrogen peroxide, thus stabilizing the immobilized enzymes.

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