High lysine maize compositions and methods for detection...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 1/00 (2006.01)

Patent

CA 2547323

Disclosed herein are assays for detecting the presence of a lysine-increasing transgenic event based on the DNA sequence of the exogenous DNA construct inserted into the maize genome and of genomic sequences flanking the insertion site. Also provided are transgenic plants having a novel exogenous DNA construct that expresses a dihydrodipicolinic acid synthase, the activity of which results in increased lysine in a plant or plant product.

L'invention porte sur des méthodes de détection de la présence d'un événement transgénique augmentant la teneur en lysine sur la base de la séquence d'ADN du produit de recombinaison d'ADN exogène inséré dans le génome du maïs et de séquences génomiques situées sur le côté du site d'insertion. L'invention porte également sur des plantes transgéniques possédant un nouveau produit de recombinaison d'ADN exogène qui exprime une synthase de l'acide dihydrodipicolinique dont l'activité entraîne une augmentation de la teneur en lysine dans une plante ou un produit végétal. .

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