High molecular weight desulphatohirudin

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/15 (2006.01) A61K 38/58 (2006.01) C07K 1/113 (2006.01) C07K 14/815 (2006.01) C12N 15/81 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2144523

The invention pertains to the field of thrombin inhibitors and describes the production of modified desulphatohirudins more especially desulphatohirudins, with the aid of genetic engineering. It is a further object of the invention to provide a method for the denaturation and renaturation of a modified desulphatohirudin to a biologically active thrombin inhibitor and the combination of two ore more of said desulphatohirudins to form higher molecular weight thrombin inhibitors.

Dans le domaine des inhibiteurs de thrombine, on a prévu la production par génie génétique de désulfatohirudines modifiées, et plus spécialement de désulfatohirudines. On a également prévu un procédé de dénaturation et de renaturation d'une désulfatohirudine modifiée afin d'obtenir un inhibiteur de thrombine à activité biologique, et d'association d'au moins deux désulfatohirudines du type précité afin de former des inhibiteurs de thrombine à poids moléculaire plus élevé.

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