G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 30/56 (2006.01) B01D 15/14 (2006.01) B01D 15/16 (2006.01) G01N 30/38 (2006.01) G01N 30/50 (2006.01) G01N 30/32 (2006.01)
Patent
CA 2236712
A method of and apparatus for liquid chromatography at improved analytical and preparative speeds and quantities involves flow of fluids through the column at flow rates sufficient to induce turbulent flow in those fluids. In one embodiment, the apparatus and method includes a substantially uniform chromatography column created by packing together a multiplicity of rigid, solid, porous particles having diameters of not less than about 30 µm, surfaces of the particles being chromatographically active. A fluid mixture containing at least one solute that is reactive with the particle surfaces is injected into the column and subsequently eluted therefrom by an eluant fluid flow, both the injection and elution being effected at a velocity sufficient to induce flow within at least a substantial portion of the interstitial volume between the particles at a reduced velocity greater than about 5,000.
L'invention porte sur un procédé et un appareil de chromatographie liquide recourant à des vitesses et des volumes d'analyse et de préparation tels qu'ils créent à travers la colonne des débits suffisants pour y produire des écoulements tourbillonnaires. Dans une variante, l'appareil et le procédé comportent une colonne de chromatographie sensiblement uniforme formée par le tassement de particules rigides, solides et poreuses de diamètres non inférieurs à environ 30 µm dont la surface est chromatographiquement active. Un mélange fluide contenant au moins un soluté réagissant avec la surface des particules est injecté dans la colonne puis en est élué par un courant de fluide éluant. L'injection et l'élution se font à une vitesse suffisante pour créer un flux dans au moins une partie substantielle du volume interstitiel entre les particules, à un vitesse réduite supérieure à environ 5000.
Quinn Hubert M.
Takarewski Joseph J. Jr.
Cohesive Technologies Inc.
Riches Mckenzie & Herbert Llp
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