High performance liquid chromatography mobile phase

Details High performance liquid chromatography mobile phase High performance liquid chromatography mobile phase

C - Chemistry, Metallurgy

07

K

252/30, 530/3.2

C07K 1/16 (2006.01) B01D 15/08 (2006.01) B01D 15/42 (2006.01)

Patent

CA 1262121

ABSTRACT OF THE INVENTION There is described a mobile phase for use in the purification of proteins using reversed phase HPLC on a preparative scale. The mobile phase is an aqueous solution containing .005 to 1M of a physiologically acceptable acid of the general formula YCOOH where Y is a polar electron withdrawing group capable of hydrogen bonding silanol groups. The solution also contains up to 95% V of a physiologically acceptable organic solvent. A preparative method of purifying proteins such as HSA or BSA using reversed phase HPLC is also described.

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