High performance microcapillary gel electrophoresis

Details High performance microcapillary gel electrophoresis High performance microcapillary gel electrophoresis

B - Operations, Transporting

01

D

204/99.02, 204/1

B01D 53/32 (2006.01) B01D 57/02 (2006.01) G01N 27/447 (2006.01)

Patent

CA 2025052

ABSTRACT A microcapillary column for high performance electro- phoresis. A preferred column includes a microcapillary, a thin layer of coating material covalently bonded to the inner surface of the microcapillary wall, and a gel comprising polyacrylamide polymerized in the tube, filling it. A thin layer of hydrophilic polymer, adsorbed on the layer of coating material and located between the coating material and gel, may also be used. The gel-containing microcapillary is prepared by covalently bonding a layer of a suitable coating material to the inner surface of the microcapillary wall, and then causing a mixture of monomer with or without crosslinking agent, initiator, and polymerization catalyst to react in the bore of the microcapillary to form a polymeric matrix. In electrophoresis, the gel-containing microcapillary provides peak efficiencies in excess of 100,000 theoretical plates and in some instances over 1,000,000 theoretical plates within separation times of less than thirty minutes, permits trace level determinations of molecular weights, and permits electrophoretic operation at fields of 300V/cm or higher, resulting in extremely high resolution separations.

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