High-performing electrophoresis gels with extended shelf lives

Details High-performing electrophoresis gels with extended shelf lives High-performing electrophoresis gels with extended shelf lives

G - Physics

01

N

G01N 27/447 (2006.01) B01D 57/02 (2006.01)

Patent

CA 2750737

Polyacrylamide gels that offer high resolution as electrophoretic media for protein separations and an improved resistance to hydrolysis upon storage are made by including either taurine, asparagine, or both as an ampholyte, in combination with either tris(hydroxymethyl)aminomethane or bis(2-hydroxyethyl)amino- tris(hydroxymethyl)methane as a buffer, plus other conventional components.

La présente invention concerne des gels de polyacrylamide qui offrent une grande résolution en tant que milieu électrophorétique pour la séparation des protéines et une résistance améliorée à l'hydrolyse lors du stockage, qui sont préparés par inclusion de taurine, d'asparagine, ou des deux en tant qu'un ampholyte, en combinaison avec du tris(hydroxyméthyl)aminométhane ou du bis(2-hydroxyéthyl)amino-tris(hydroxyméthyl)méthane en tant que tampon, ainsi que d'autres composants conventionnels.

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