C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/09 (2006.01) C12N 7/00 (2006.01)
Patent
CA 2624242
The invention relates to a method for producing an infectious hepatitis C virus (HCV) particle, comprising the step of introducing an expression vector containing a DNA fragment including a DNA sequence encoding a 5~ noncoding region and a structural protein of HCV and an arbitrary nonstructural protein and a DNA sequence encoding a nonstructural protein and a 3~ noncoding region derived from HCV JFH1 strain downstream of the RNA polymerase I promoter and further containing a DNA fragment including the RNA polymerase I terminator downstream thereof into a cell which allows HCV proliferation.
L'invention concerne un procédé de production d'une particule du virus de l'hépatite C infectieuse (HCV), lequel comprend l'étape consistant à introduire un vecteur d'expression contenant un fragment d'ADN comportant une séquence d'ADN codant une région non codante 5' et une protéine structurelle de HCV et une protéine non structurelle aléatoire et une séquence d'ADN codant une protéine non structurelle et une région non codante 3' dérivée d'une souche JFH1 HCV en aval du promoteur de polymérase I d'ARN et contenant également un fragment d'ADN comprenant l'agent de terminaison de polymérase I d'ARN en aval de celui-ci dans une cellule qui permet la prolifération de HCV.
Ishii Koji
Miyamura Tatsuo
Sone Saburo
Suzuki Ryosuke
Suzuki Tetsuro
Japan As Represented By Director-General Of National Institute O
Smart & Biggar
Tokyo Metropolitan Institute Of Medical Science
Tokyo Metropolitan Organization For Medical Research
Toray Industries Inc.
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