High resolution dna detection methods and devices

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) B01L 7/00 (2006.01) G01N 27/00 (2006.01) H01L 51/30 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2367405

The present invention provides methods and devices for detecting a target nucleic acid molecule. A set of oligonucleotide probes integrated into an electric circuit, where the oligonucleotide probes are positioned such that they can not come into contact with one another, are contacted with a sample. If the sample contains a target nucleic acid molecule, one which has sequences complementary to both probes, the target nucleic acid molecule can bridge the gap between the probes. The resulting bridge can then carry electrical current between the two probes, indicating the presence of the target nucleic acid molecule.

La présente invention concerne des procédés et des dispositifs permettant de détecter une molécule d'acide nucléique cible. Un ensemble de sondes d'oligonucléotides intégrées dans un circuit électrique, dans lequel ces sondes d'oligonucléotides sont positionnées de telle sorte qu'elles ne peuvent pas entrer en contact les unes avec les autres, est mis en contact avec un échantillon. Lorsque cet échantillon contient une molécule d'acide nucléique cible, qui comprend des séquences complémentaires de ces deux sondes, la molécule d'acide nucléique cible peut combler l'espace entre les sondes. Le fait de combler cet espace permet de véhiculer un courant électrique entre les deux sondes, ce qui indique la présence d'une molécule d'acide nucléique cible.

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