High resolution dna sequencing method using low viscosity...

Details High resolution dna sequencing method using low viscosity... High resolution dna sequencing method using low viscosity...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) G01N 27/26 (2006.01) G01N 27/447 (2006.01)

Patent

CA 2153991

A DNA sequencing method for use in sequencing a DNA target sequence up to 300 bases, preferably up to 500 bases or greater in length, by electrophoretically separating a mixture of single-stranded DNA sequencing fragments in a capillary tube. The method employs an aqueous denaturing solution comprising between about 4 and about 7 weight percent linear polyacrylamide molecules having an average molecular weight of between about 20 and about 100 kilodaltons. The low-viscosity of the solution allows rapid loading and reloading of such solution into the capillary tube.

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