High resolution methods and precipitating reagents for...

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 1/30 (2006.01)

Patent

CA 2603551

Novel high resolution methods and precipitating reagents for isolating desired protein populations from proteinaceous materials are disclosed. Uniquely, the methods and precipitating reagents of this invention are suitable for selectively and sequentially isolating consistently in high yields desired protein populations from various proteinaceous materials based upon the physical and chemical characteristics inherent in, and/or peculiar to, the desired protein populations. In addition, desired protein populations isolated with the new and vastly improved methods and precipitating reagents of this invention are highly concentrated and substantially pure and biologically active. The precipitating reagents basically are an alkali metal salt of a polyfunctional organic acid, exemplified by tri-potassium citrate monohydrate or tri-sodium citrate dihydrate, and a buffer for adjusting pH. Sources of proteinaceous materials may include, for example, animal or vegetable fluids and extracts.

L'invention porte sur de nouveaux procédés et réactifs de précipitation permettant d'isoler des populations de protéines recherchées à partir de matériaux protéiques, et de manière unique, d'isolement sélectif, séquentiel et régulièrement à haut rendement de populations de protéines recherchées à partir de différents matériaux protéiques, sur la base de propriétés physiques et chimiques inhérentes et/ou particulières auxdites populations. De plus grâce à ces procédés et réactifs de précipitation largement améliorés, lesdites populations sont extrêmement concentrées, quasiment pures et biologiquement actives. Les réactifs de précipitation sont fondamentalement un sel de métal alcalin d'un acide organique polyfonctionnel, par exemple un citrate de tripotassium monihydraté ou un citrate de trisodium dihydraté, auquel on ajoute un tampon d'ajustement du pH. Les sources de matériaux protéiques peuvent par exemple être des fluides et extraits animaux ou végétaux.

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