High salt concentration to prolong light signal in...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/66 (2006.01)

Patent

CA 2345721

The invention relates to a method for detecting ATP in a sample, wherein the sample is contacted with a reaction mixture to effect a light signal, the reaction mixture comprising luciferin, luciferase and one or more water- soluble salts, the total salt concentration being at leat 0.05 mole/liter, and wherein the light signal is measured. The invention further relates to a kit for use in this method.

L'invention concerne une méthode de détection d'ATP dans un prélèvement, la méthode consistant à mettre en contact le prélèvement avec un mélange réactionnel pour produire un signal lumineux, le mélange réactionnel comprenant de la luciférine, de la luciférase et un ou plusieurs sels solubles dans l'eau et la concentration totale en sel s'élevant à au moins 0,05 moles/litre; puis à mesurer le signal lumineux. L'invention concerne en outre une trousse destinée à s'utiliser avec cette méthode.

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