High sensitivity phage display biomolecule detection

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/02 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2368875

The invention relates to a biomolecular complex comprising a target biomolecule; a phage having a phage-expressed binding protein that can bind to the target biomolecule or having joined thereto a linked or un-linked protein or nucleic acid that can bind to the target biomolecule, wherein the phage- expressed binding protein or the linked or unlinked protein or nucleic acid is bound to the target biomolecule to provide a bound phage; and a host bacterial cell that is a host for the phage under conditions that permit the bound phage to infect the host.

L'invention concerne un complexe biomoléculaire, comprenant une biomolécule cible; un phage exprimant une protéine de liaison qui peut se lier à ladite biomolécule cible ou possédant une protéine liée ou non liée ou un acide nucléique qui peuvent se lier à la biomolécule cible, la protéine de liaison exprimée par le phage, la protéine liée ou non liée, ou l'acide nucléique étant liés à la biomolécule cible afin d'obtenir un phage de liaison; et une cellule bactérienne hôte du phage dans des conditions qui permettent à un phage de liaison d'infecter ledit hôte.

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