High specificity primers, amplification methods and kits

Details High specificity primers, amplification methods and kits High specificity primers, amplification methods and kits

C - Chemistry, Metallurgy

07

H

C07H 21/04 (2006.01) C07H 21/00 (2006.01) C07H 21/02 (2006.01) C12N 15/09 (2006.01) C12P 19/34 (2006.01) C12Q 1/48 (2006.01) C12Q 1/68 (2006.01) G01N 33/00 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2375027

For nucleic acid amplification including extension of primers by a DNA polymerase, high specificity primers are provided. The primers include a type of hairpin structure in which a single- stranded loop separates complementary 3' and 5' arms and in which the loop and the 3' arm are complementary to the target nucleic acid. Amplification methods, assays and kits including such primers are included in the invention.

La présente invention concerne une amplification d'acide nucléique comprenant le déploiement d'amorces par une polymérase d'ADN, et des amorces hautement spécifiques. Ces amorces comprennent un type de structure en épingle à cheveux dans laquelle une boucle à un seul brin sépare des branches complémentaires 3' et 5', et dans laquelle la boucle et la branche 3' sont complémentaires de l'acide nucléique cible. Cette invention concerne aussi des techniques d'amplification, des analyses et des kits comprenant ces amorces.

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