High speed, high resolution compositions, methods, and kits...

G - Physics – 01 – N

Patent

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G01N 27/447 (2006.01) B01D 53/02 (2006.01)

Patent

CA 2422971

The invention provides compositions, methods and kits for high speed, high resolution of analytes by capillary electrophoresis starting with uncoated capillaries. The compositions comprise a sieving component, comprising a non- crosslinked acrylamide polymer, and a surface interaction component, comprising at least one uncharged and non-crosslinked water-soluble silica- adsorbing polymer. Methods for employing the novel compositions in capillary electrophoresis are provided. Kits comprising the novel compositions for use in the novel methods are also provided.

L'invention concerne des compositions, des procédés et des trousses destinés à une haute résolution à haute sensibilité d'analytes par électrophorèse capillaire commençant par des capillaires non revêtus. Les compositions comportent un composant de criblage, contenant un polymère acrylamide non réticulé, et un composant d'interaction de surface, contenant au moins un polymère adsorbant de silice hydrosoluble non réticulé. L'invention traite également de procédé utilisant en électrophorèse capillaire les nouvelles compositions, ainsi que de trousses comprenant ces nouvelles compositions pouvant être servir dans ces procédés.

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